Cholesterol Assay Kit - HDL and LDL/VLDL - labmallx.com

Key features and details

Assay type: Quantitative
Detection method: Colorimetric/Fluorometric
Platform: Microplate reader
Assay time: 1 hr
Sample type: Other biological fluids, Plasma, Serum, Tissue Lysate, Urine

Overview

Product name

Cholesterol Assay Kit – HDL and LDL/VLDL
See all HDL LDL/VLDL kits

Detection method

Colorimetric/Fluorometric

Sample type

Urine, Serum, Plasma, Other biological fluids, Tissue Lysate

Assay type

Quantitative

Assay time

1h 00m

Product overview

Cholesterol Assay Kit ab65390 uses a simple method to quantify total cholesterol, free cholesterol, and cholesterol esters in mammalian samples. It also includes an easy method to separate HDL and LDL / VLDL cholesterol.

In the cholesterol assay protocol, cholesterol oxidase acts on free cholesterol to produce a chemical which reacts with a probe to generate color (570 nm) and fluorescence (Ex/Em = 538/587 nm). Cholesterol esterase is used to hydrolyze cholesteryl ester into free cholesterol.

If cholesterol esterase is included in the assay, total cholesterol is measured. If it is not included, free cholesterol is measured. The amount of cholesterol ester can be calculated by subtracting free cholesterol from total cholesterol.

Properties

Storage instructions

Store at -20°C. Please refer to protocols.

Components	Identifier	100 tests
2X LDL/VLDL Precipitation Buffer	NW	1 x 10ml
Cholesterol Assay Buffer	WM	1 x 25ml
Cholesterol Esterase (Lyophilized)	Blue	1 vial
Cholesterol Probe (in DMSO, anhydrous)	Red	1 x 200µl
Cholesterol Standard (2 ug/ul)	Yellow	1 x 100µl
Enzyme Mix (Lyophilized)	Green	1 vial

Research areas

Cardiovascular
Lipids / Lipoproteins
Lipid Metabolism
Cholesterol Metabolism

Kits/ Lysates/ Other
Kits
Cell Metabolism Kits
Other Metabolism Assay

Kits/ Lysates/ Other
Kits
Cell Metabolism Kits
Lipid Metabolism Kits
Cholesterol

Kits/ Lysates/ Other
Kits
Cell Metabolism Kits
Lipid Metabolism Kits
Obesity

Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Cholesterol Metabolism

Metabolism
Types of disease
Metabolic disorders

Metabolism
Pathways and Processes
Redox metabolism
Fatty acid oxidation

Relevance

HDL (high-density-lipoprotein), LDL (low-density lipoprotein) and VLDL (very low-density lipoprotein) are lipoproteins that act as carrier proteins for cholesterol. The regulation of HDL-cholesterol and LDL-cholesterol plays a central role in various disease development. It is well known that low levels of HDL and high level of LDL are associated with an increased risk of cardiovascular events.