8 isoprostane ELISA Kit

SKU: N/A Categories: ,

Key features and details

  • Sensitivity: 1 pg/ml
  • Range: 0.005 ng/ml – 5 ng/ml
  • Sample type: Cell culture extracts, Plasma, Serum, Tissue, Urine
  • Detection method: Colorimetric
  • Assay type: Competitive

Overview

Product name

8 isoprostane ELISA Kit

Detection method

Colorimetric

Precision

Intra-assay
SamplenMeanSDCV%
Overall12  0.43%
Inter-assay
SamplenMeanSDCV%
Overall12  1.75%

Sample type

Urine, Serum, Plasma, Cell culture extracts, Tissue

Assay type

Competitive

Sensitivity

1 pg/ml

Range

0.005 ng/ml – 5 ng/ml

Assay duration

Multiple steps standard assay

Product overview

Abcam’s 8 isoprostane competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the determination of 8 isoprostane levels in biological samples. The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissues phospholipids by oxygen radicals. A recent NIH-sponsored study on Biomarkers of Oxidative Stress has indicated that 8 isoprostane is the best index of oxidative injury in a well-accepted oxidant stress rat model. In addition, plasma 8 isoprostane levels were found to be elevated in elderly subjects with severe hypertension and in urine from subjects with high fat diet-induced liver steatosis.

This kit can be used for the determination of 8 isoprostane in diluted urine, serum, plasma, cells and tissues following proper isolation and purification of the eicosanoid from the isoprostane-containing sample.

 

Platform

Microplate

Properties

Storage instructions

Store at -20°C. Please refer to protocols.
Components1 x 96 tests
1,000X 8-isoprostane HRP Conjugates1 x 12µl
10X Sample Dilution Buffer1 x 25ml
10X Wash Buffer Solution1 x 25ml
8-isoprotane Standard1 x 2µl
HRP Buffer1 x 15ml
Pre-coated 96-Well Microplate1 unit
TMB Substrate1 x 22ml

Research areas

  • Cell Biology
  •  
  • Other Antibodies
  •  
  • Oxidative Stress
  • Metabolism
  •  
  • Pathways and Processes
  •  
  • Redox metabolism
  •  
  • Oxidative stress

Alternative names

  • Prostaglandin, iso Prostaglandin F2, iso Prostaglandin, iPF2 alpha

Protocols