Human ACE2 ELISA Kit

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Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1052 pg/ml
  • Range: 3.98 ng/ml – 255 ng/ml
  • Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

Product name

Human ACE2 ELISA Kit
See all ACE2 kits

Detection method

Colorimetric

Precision

Intra-assay
SamplenMeanSDCV%
extract8  2.3%
Inter-assay
SamplenMeanSDCV%
extract3  3.2%

Sample type

Urine, Serum, Tissue Extracts, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

Assay type

Sandwich (quantitative)

Sensitivity

1052 pg/ml

Range

3.98 ng/ml – 255 ng/ml

Recovery

 

Sample specific recovery
Sample typeAverage %Range
Urine8885% – 89%
Serum99% – %
Tissue Extracts104100% – 108%
Cell culture media112110% – 116%
Hep Plasma8281% – 84%
EDTA Plasma9190% – 92%
Cit plasma9998% – 100%

Assay time

1h 30m

Assay duration

One step assay

Species reactivity

Reacts with: Human

Product overview

Human ACE2 ELISA Kit (ab235649) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of native ACE2 protein in tissue extracts and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ACE2 with 1052 pg/ml sensitivity.


Note: Plasma, serum, and cell culture media samples were validated with spiked recombinant ACE2. Neat serum from ten individual healthy human female/male donors was measured in duplicate and all values were below the detectable range of the assay.


SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


        – Single-wash protocol reduces assay time to 90 minutes or less
        – High sensitivity, specificity and reproducibility from superior antibodies
        – Fully validated in biological samples
        – 96-wells plate breakable into 12 x 8 wells strips


A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Notes

ACE2 is a key enzyme in the renin-angiotensin system which regulates blood pressure. Specifically, ACE2 cleaves the terminal nonapeptide from angiotensin I or the terminal septapeptide from angiotensin II to create angiotensin[1-9] and angiotensin[1-7], respectively. The produced peptides work to oppose the effects of angiotensin II and act as a vasodilator. This enzyme is currently under investigation as a potential target for reducing instances of heart failure.

 

Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Platform

Pre-coated microplate (12 x 8 well strips)

Properties

Storage instructions

Store at +4°C. Please refer to protocols.
Components1 x 96 tests
10X Human ACE2 Capture Antibody1 x 600µl
10X Human ACE2 Detector Antibody1 x 600µl
10X Wash Buffer PT (ab206977)1 x 20ml
50X Cell Extraction Enhancer Solution (ab193971)1 x 1ml
5X Cell Extraction Buffer PTR (ab193970)1 x 10ml
Antibody Diluent 4BI1 x 6ml
Human ACE2 Lyophilized Recombinant Protein2 vials
Plate Seals1 unit
Sample Diluent NS (ab193972)1 x 12ml
SimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unit
Stop Solution1 x 12ml
TMB Development Solution1 x 12ml

Research areas

  • Microbiology
  •  
  • Organism
  •  
  • Virus
  •  
  • RNA Virus
  •  
  • ssRNA positive strand virus
  •  
  • SARS Coronavirus
  • Cell Biology
  •  
  • Proteolysis / Ubiquitin
  •  
  • Proteolytic enzymes
  •  
  • Metalloprotease
  •  
  • ACEs
  • Cardiovascular
  •  
  • Blood
  •  
  • Blood Pressure regulation
  • Cardiovascular
  •  
  • Heart
  •  
  • Contractility
  •  
  • Inotropics
  • Cardiovascular
  •  
  • Vasculature
  •  
  • Vasoconstriction
  •  
  • Other

Function

Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.

Tissue specificity

Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.

Sequence similarities

Belongs to the peptidase M2 family.

Post-translational
modifications

N-glycosylation on Asn-90 may limit SARS infectivity.

Cellular localization

Secreted and Cell membrane.

Protocols