Corticosterone ELISA kit

SKU: N/A Categories: ,

Key features and details

  • Sensitivity: 0.32 ng/ml
  • Range: 0.18 ng/ml – 19.7 ng/ml
  • Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Competitive
  • Reacts with: Mouse, Rat, Human, Baboon


Product name

Corticosterone ELISA kit

Detection method



Overall   6.3%
Overall   10.6%

Sample type

Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma

Assay type



= 0.32 ng/ml


0.18 ng/ml – 19.7 ng/ml


= 101 %

Assay time

3h 00m

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Mouse, Rat, Human, Baboon

Product overview

Corticosterone ELISA  kit is a competitive Enzyme-Linked Immunosorbent Assay designed for the quantitative measurement of Corticosterone levels in plasma, serum, urine, milk, saliva and cell culture supernatant.

A Corticosterone specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Corticosterone is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Corticosterone captured in plate.

Please note the concentrations of the kit components are lot-specific and the end user should always refer to the vial label

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.




Storage instructions

Store at -20°C. Please refer to protocols.
Components1 x 96 tests
100X Streptavidin-Peroxidase Conjugate1 x 80µl
10X Diluent S Concentrate1 x 20ml
1X Biotinylated Corticosterone Protein (Lyophilized)1 vial
20X Wash Buffer Concentrate1 x 30ml
Chromogen Substrate1 x 7ml
Corticosterone Microplate (12 x 8 well strips)1 unit
Corticosterone Standard1 vial
Sealing Tapes3 units
Stop Solution1 x 11ml