Human TACE ELISA Kit (ADAM17)

SKU: N/A Categories: ,

Key features and details

  • Sensitivity: 70 pg/ml
  • Range: 78.15 pg/ml – 5000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human TACE ELISA Kit (ADAM17)
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 70 pg/ml
  • Range

    78.15 pg/ml – 5000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample typeAverage %Range
    Cell culture supernatant98.6589% – 124%
    Serum112.385% – 132%
    Plasma101.172% – 128%
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s TACE Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TACE in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human TACE coated on a 96-well plate. Standards and samples are pipetted into the wells and TACE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TACE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TACE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

    The standard is affinity purified.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • ComponentsIdentifier1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Human TACEItem F2 vials
    Recombinant Human TACE Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TACE Microplate (12 x 8 wells)Item A1 unit
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

    • Cell Biology
    •  
    • Apoptosis
    •  
    • Receptors
    •  
    • Associated Proteins
    • Cell Biology
    •  
    • Cell Cycle
    •  
    • Cell differentiation
    • Signal Transduction
    •  
    • Cytoskeleton / ECM
    •  
    • Extracellular Matrix
    •  
    • ECM Enzymes
    •  
    • ADAM Protein Family
    • Cancer
    •  
    • Invasion/microenvironment
    •  
    • ECM
    •  
    • Extracellular matrix
    •  
    • ADAM protein family
    • Cell Biology
    •  
    • Proteolysis / Ubiquitin
    •  
    • Proteolytic enzymes
    •  
    • Metalloprotease
    •  
    • ADAMs
    • Kits/ Lysates/ Other
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    • Cancer
    •  
    • Cell Death
    •  
    • Apoptosis
    •  
    • Receptors
    •  
    • Associated Proteins

Protocols

Available Options

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