Human IL-6 ELISA Kit

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Key features and details

  • Sensitivity: 2 pg/ml
  • Range: 6.25 pg/ml – 200 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

Product name

Human IL-6 ELISA Kit

Detection method

Colorimetric

Precision

Intra-assay
SamplenMeanSDCV%
Pooled sampl15  4.2%
Inter-assay
SamplenMeanSDCV%
Sample 118200pg/ml168.1%
Sample 21877pg/ml810.1%
Sample 31863pg/ml68.6%
Sample 418103pg/ml66%
Sample 51853pg/ml35.5%

Sample type

Cell culture supernatant, Serum, Plasma

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/ml

Range

6.25 pg/ml – 200 pg/ml

Recovery

96 %

Assay time

1h 45m

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Human

Product overview

Abcam’s IL-6 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 in Human serum, plasma, buffered solutions or supernatants.


A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples.


This kit will recognize both endogenous and recombinant Human IL-6.


Get higher sensitivity in only 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.

Platform

Microplate

Properties

Storage instructions

Store at +4°C. Please refer to protocols.
ComponentsIdentifier1 x 96 tests2 x 96 tests
10X Standard Diluent BufferBlack1 x 15ml1 x 25ml
200X Wash BufferWhite1 x 10ml2 x 10ml
Adhesive plate seal 1 x 2 units1 x 4 units
Biotinylated Antibody DiluentRed1 x 7.5ml1 x 13ml
Biotinylated anti-IL-6Red1 x 0.4ml2 x 0.4ml
Chromogen TMB Substrate Solution 1 x 11ml1 x 24ml
HRP DiluentRed1 x 12ml1 x 23ml
IL-6 ControlYellow2 vials4 vials
IL-6 Microplate (12 x 8 well strips) 1 unit2 units
IL-6 Standard (Lyophilized)Orange2 vials4 vials
Standard Diluent (Serum) 1 x 7ml2 x 7ml
Stop ReagentBlack1 x 11ml2 x 11ml
Streptavidin-HRP 2 x 5µl4 x 5µl

Research areas

  • Immunology
  •  
  • Innate Immunity
  •  
  • Macrophage / Inflamm.
  • Immunology
  •  
  • Innate Immunity
  •  
  • Cytokines
  •  
  • Interleukins
  • Neuroscience
  •  
  • Neurology process
  •  
  • Metabolism
  • Microbiology
  •  
  • Organism
  •  
  • Virus
  •  
  • RNA Virus
  •  
  • ssRNA positive strand virus
  •  
  • SARS Coronavirus
  • Cancer
  •  
  • Invasion/microenvironment
  •  
  • Angiogenesis
  •  
  • Angiogenic growth factors
  • Cancer
  •  
  • Tumor immunology
  •  
  • Cytokines
  •  
  • Interleukins
  • Kits/ Lysates/ Other
  •  
  • Kits
  •  
  • ELISA Kits
  •  
  • ELISA Kits
  •  
  • Cytokines and cytokine receptors ELISA kits
  • Metabolism
  •  
  • Types of disease
  •  
  • Obesity
  • Metabolism
  •  
  • Types of disease
  •  
  • Metabolic disorders
  • Neuroscience
  •  
  • Processes

Function

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

Involvement in disease

Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.

Sequence similarities

Belongs to the IL-6 superfamily.

Post-translational
modifications

N- and O-glycosylated.

Cellular localization

Secreted.

Protocols

Available Options

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