HMG-CoA Reductase Activity Assay Kit (Colorimetric)

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Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 10 min
  • Sample type: Purified protein

Overview

  • Product name

    HMG-CoA Reductase Activity Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Purified protein
  • Assay type

    Enzyme activity
  • Assay time

    0h 10m
  • Product overview

    HMG-CoA Reductase Activity Assay Kit (Colorimetric) (ab204701) is suitable for measuring activity of purified HMG-CoA reductase or for screening inhibitors/activators of HMG-CoA reductase. It is based on the consumption of NADPH by the enzyme, which can be measured by the decrease of absorbance at OD=340 nm. The limit of detection is below 0.05 mU.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K588 H mg-CoA Reductase Activity/Inhibitor Screening Kit (Colorimetric). K588-100 is the same size as the 100 test size of ab204701.

    HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGR) (EC 1.1.1.34) is the rate-controlling enzyme of the mevalonate pathway, the metabolic pathway that produces cholesterol from acetyl-CoA. In an NADPH-dependent reaction, HMG-CoA reductase reduces HMG-CoA to generate mevalonate and CoA. The enzyme is target of a group of cholesterol-lowering drugs known as statins. Inhibition of HMG-CoA reductase induces expression of LDL receptors in the liver, which lowers plasma concentration of cholesterol.

  • Platform

    Microplate reader

Properties

Storage instructions

Store at -20°C. Please refer to protocols.
ComponentsIdentifier100 tests
HMG-CoA Reductase Assay Buffer 
HMG-CoA Reductase 
HMG-CoA 
NADPH 
Inhibitor (Atorvastatin, 10 mM) 

Research areas

  • Cardiovascular
  •  
  • Lipids / Lipoproteins
  •  
  • Lipid Metabolism
  •  
  • Cholesterol Metabolism
  • Signal Transduction
  •  
  • Metabolism
  •  
  • Lipid metabolism
  • Cardiovascular
  •  
  • Atherosclerosis
  •  
  • Lipoprotein metabolism
  • Cancer
  •  
  • Cancer Metabolism
  •  
  • Metabolic signaling pathway
  •  
  • Metabolism of lipids and lipoproteins
  • Metabolism
  •  
  • Pathways and Processes
  •  
  • Metabolic signaling pathways
  •  
  • Lipid and lipoprotein metabolism
  •  
  • Lipid metabolism
  • Metabolism
  •  
  • Pathways and Processes
  •  
  • Metabolic signaling pathways
  •  
  • Lipid and lipoprotein metabolism
  •  
  • Cholesterol Metabolism
  • Metabolism
  •  
  • Pathways and Processes
  •  
  • Metabolic signaling pathways
  •  
  • Lipid and lipoprotein metabolism
  •  
  • Lipoprotein metabolism
  • Metabolism
  •  
  • Types of disease
  •  
  • Heart disease

Function

This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis.

Pathway

Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.

Sequence similarities

Belongs to the HMG-CoA reductase family.

Cellular localization

Endoplasmic reticulum membrane. Peroxisome membrane.

 

Protocols