Human Anti-Trichinella spiralis IgG ELISA Kit

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Key features and details

  • Sample type: Cit plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Indirect
  • Reacts with: Human

Overview

Product name

Human Anti-Trichinella spiralis IgG ELISA Kit

Detection method

Colorimetric

Precision

Intra-assay
SamplenMeanSDCV%
Pos. Serum8  3.9%
Inter-assay
SamplenMeanSDCV%
Pos. Serum4  5.2%

Sample type

Serum, Hep Plasma, Cit plasma

Assay type

Indirect

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Human

Product overview

Abcam’s anti-Trichinella spiralis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Trichinella spiralis in Human serum and plasma.


A 96-well plate has been precoated with Trichinella spiralis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Trichinella spiralis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Trichinella spiralis IgG sample captured in plate.

Platform

Microplate

Properties

Storage instructions

Store at +4°C. Please refer to protocols.
ComponentsIdentifier1 x 96 tests
20X Washing SolutionWhite cap1 x 50ml
Cover foil 1 unit
IgG Sample DiluentColored yellow; White cap1 x 100ml
Stop Solutionred cap1 x 15ml
Strip holder 1 unit
TMB Substrate SolutionYellow cap1 x 15ml
Trichinella spiralis (IgG) Coated Microplate (12 x 8 wells)12 strips of 8 wells1 unit
Trichinella spiralis IgG Cut-off ControlColored yellow; Green cap1 x 3ml
Trichinella spiralis IgG Negative ControlColored yellow; Blue cap1 x 2ml
Trichinella spiralis IgG Positive ControlColored yellow; Red cap1 x 2ml
Trichinella spiralis Protein A HRP ConjugateColored blue; Black cap1 x 20ml

Research areas

  • Immunology
  •  
  • Immunoglobulins
  •  
  • Heavy Chain
  •  
  • IgG
  • Microbiology
  •  
  • Organism
  •  
  • Eukaryotic Microorganism
  •  
  • Metazoa
  •  
  • Nematoda
  • Kits/ Lysates/ Other
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  • ELISA Kits
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  • Pathogen specific immunoglobulins ELISA kits
  • Kits/ Lysates/ Other
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  • Infectious disease ELISA kits

Protocols