Human C Reactive Protein ELISA Kit (PTX1)

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Key features and details

  • Sensitivity: 0.2 ng/ml
  • Range: 0.25 ng/ml – 16 ng/ml
  • Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human C Reactive Protein ELISA Kit (PTX1)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    SamplenMeanSDCV%
    Overall   4%
    Inter-assay
    SamplenMeanSDCV%
    Overall   11.3%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.2 ng/ml
  • Range

    0.25 ng/ml – 16 ng/ml
  • Recovery

    96 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s C Reactive Protein (PTX1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C Reactive Protein concentrations in Human plasma, serum, saliva, milk, urine and cell culture supernatants.


    A C Reactive Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a C Reactive Protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of C Reactive Protein captured in plate.


    Get results in 90 minutes with Human C Reactive Protein ELISA Kit (ab181416) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate1 x 80µl
    10X Diluent N Concentrate1 x 30ml
    20X Wash Buffer Concentrate2 x 30ml
    50X Biotinylated Human C Reactive Protein Antibody1 x 120µl
    C Reactive Protein Microplate (12 x 8 well strips)1 unit
    C Reactive Protein Standard1 vial
    Chromogen Substrate1 x 8ml
    Sealing Tapes3 units
    Stop Solution1 x 12ml
  • Research areas

    • Immunology
    •  
    • Innate Immunity
    •  
    • Macrophage / Inflamm.
    • Cardiovascular
    •  
    • Blood
    •  
    • Acute Phase Reactants
    • Immunology
    •  
    • Innate Immunity
    •  
    • Complement
    •  
    • Classical Pathway
    • Epigenetics and Nuclear Signaling
    •  
    • Chromatin Binding Proteins
    •  
    • DNA / RNA binding
    • Cardiovascular
    •  
    • Atherosclerosis
    •  
    • Vascular Inflammation
    •  
    • Inflammatory mediators
    • Cardiovascular
    •  
    • Heart
    •  
    • Apoptosis
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    • Metabolism
    •  
    • Types of disease
    •  
    • Metabolic disorders
    • Neuroscience
    •  
    • Processes
  • Function

    Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
  • Tissue specificity

    Found in plasma.
  • Sequence similarities

    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Cellular localization

    Secreted.

Protocols

Available Options

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