beta-Amyloid Peptide (1-42) (human)

Key features and details

beta-Amyloid (1-42) protein fragment. Implicated in Alzheimer’s disease.
CAS Number: 107761-42-2
Solubility is batch-dependent. Please refer to the Protocol Booklet and the batch-specific CoA for more information.
Form / State: Solid
Source: Synthetic

Overview

Product name

beta-Amyloid Peptide (1-42) (human)

Description

beta-Amyloid (1-42) protein fragment. Implicated in Alzheimer’s disease.

Alternative names

AB42

CAS Number

107761-42-2

Chemical structure

Properties

Molecular weight

4514.08

Molecular formula

C₂₀₃H₃₁₁N₅₅O₆₀S

Sequence

DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

PubChem identifier

57339251

Storage instructions

Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.

Solubility overview

Solubility is batch-dependent. Please refer to the Protocol Booklet and the batch-specific CoA for more information.

Handling

This product is supplied in one (or more) pack size which is freeze dried. Therefore the contents may not be readily visible, as they can coat the bottom or walls of the vial. Please see our FAQs and information page for more details on handling.

Solvents listed may be unsuitable for use in biological experiments. These solvents are intended to enable solubilisation and mixing of components. We recommend that biologically unsuitable solvents are removed prior to solubilisation in experimental media.

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Amyloid β (1-42) human peptide should be initially dissolved at a concentration of 1mg/ml in 100% HFIP (1,1,1,3,3,3-hexafluoro-2-propanol). This solution should be incubated at room temperature for 1 hour, with occasional vortexing at a moderate speed. Next, the solution should be sonicated for 10 minutes in a water bath sonicator. The HFIP/peptide solution should then be dried under a gentle stream of nitrogen gas. 100% DMSO should be used to re-suspend the peptide. This solution should be incubated at room temperature for 12 minutes, with occasional vortexing. The final solution should then be aliquoted into smaller volumes and stored at -80 °C. For a working solution, add 500-1000 μl of D-PBS (depending on the final concentration to be used) to the peptide stock solution and incubate for 2h at room temperature to allow for peptide aggregation.

Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.