Total Antioxidant Capacity Assay Kit

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Key features and details

  • Assay type: Quantitative
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 2 hr
  • Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine

Overview

  • Product name

    Total Antioxidant Capacity Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type

    Quantitative
  • Assay time

    2h 00m
  • Product overview

    Total Antioxidant Capacity Assay Kit ab65329 can measure either the combination of both small molecule antioxidants and proteins, or small molecules alone in the presence of our proprietary Protein Mask.


    In the total antioxidant capacity assay protocol, the Cu2+ ion is converted to Cu+ by both small molecule and protein antioxidants. The Protein Mask prevents Cu2+ reduction by proteins, enabling the analysis of only the small molecule antioxidants. The reduced Cu+ ion is chelated with a colorimetric probe giving a broad absorbance peak around 570 nm, proportional to the total antioxidant capacity.


    Total antioxidant capacity assay protocol summary:
    – add protein mask to samples if only measuring small molecule total antioxidant capacity
    – add samples and standards to wells
    – add Cu2+ solution and incubate for 90 min at room temp
    – analyze with microplate reader

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K274 Total Antioxidant Capacity (TAC) Colorimetric Assay Kit. K274-100 is the same size as the 100 test size of ab65329.

    Antioxidants play an important role in preventing the formation of, and scavenging of, free radicals and other oxidizing species. There are three categories of antioxidant species: enzyme systems (GSH reductase, catalase, peroxidase, etc.), small molecules (ascorbate, uric acid, GSH, vitamin E, etc.) and proteins (albumin, transferrin, etc.). 

    Trolox is used to standardize antioxidants, with all other antioxidants being measured in Trolox equivalents. Measurement of the combined non-enzymatic antioxidant capacity of biological fluids and other samples provides an indication of the overall capability to counteract reactive oxygen species (ROS), resist oxidative damage and combat oxidative stress-related diseases.

    Related products

    Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • ComponentsIdentifier100 tests
    Assay DiluentWM1 x 10ml
    Cu++ ReagentBlue1 x 0.2ml
    Protein MaskNM1 x 10ml
    Trolox Standard (1 µmol) (Lyophilized)Yellow1 vial
  • Research areas

    • Kits/ Lysates/ Other
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    • Kits
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    • Cell Damage Kits
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    • Oxidative stress
    • Kits/ Lysates/ Other
    •  
    • Kits
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    • Cell Damage Kits
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    • Cell Damage
    • Kits/ Lysates/ Other
    •  
    • Kits
    •  
    • Cell Metabolism Kits
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    • Oxidative Stress Assay Kits
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    • Oxidative Stress
    • Kits/ Lysates/ Other
    •  
    • Kits
    •  
    • Cell Damage Kits
    •  
    • Antioxidant
  • Relevance

    Antioxidants play an important role in preventing the formation of and scavenging of free radicals and other potentially toxic oxidizing species. There are three categories of antioxidant species: enzyme systems (GSH reductase, catalase, peroxidase, etc.), small molecules (ascorbate, uric acid, GSH, vitamin E, etc.) and proteins (albumin, transferrin, etc.). Different antioxidants vary in their reducing power, and cooperation of all different antioxidants provides greater protection against reactive oxigen or nitrogen radicals than any single compound alone. Therefore, the overall total antioxidant capacity may give more relevant biological information compared to that obtained by the measurement of individual components, as it considers the cumulative effect of all antioxidants present.

Protocols

Available Options

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