Human FOXP3 ELISA Kit

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Key features and details

  • Sensitivity: 0.188 ng/ml
  • Range: 0.313 ng/ml – 20 ng/ml
  • Sample type: Plasma, Serum, Tissue Homogenate
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human FOXP3 ELISA Kit
    See all FOXP3 kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 0.188 ng/ml
  • Range

    0.313 ng/ml – 20 ng/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    This FOXP3 ELISA kit (ab285290, K4224) is a sandwich ELISA assay for the quantitative measurement of human FOXP3 in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of FOXP3 captured from the samples.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K4224 FOXP3 (Human) ELISA Kit. K4224-100 is the same size as the 96 test size of ab285290.

  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components96 tests
    25X Wash buffer1 x 30ml
    Antibody dilution buffer1 x 10ml
    Biotin-Detection antibody (Concentrated)1 x 120µl
    HRP-Streptavidin Conjugate (SABC)1 x 120µl
    Lyophilized Standard2 vials
    Micro ELISA Plate1 unit
    Plate Sealer5 units
    SABC dilution buffer1 x 10ml
    Sample and Standard Dilution Buffer1 x 20ml
    Stop Solution1 x 10ml
    TMB Substrate1 x 10ml
  • Research areas

    • Cell Biology
    •  
    • Cell Cycle
    •  
    • Cell Division
    •  
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    •  
    • Transcription
    •  
    • Domain Families
    •  
    • Forkhead Box
    •  
    • FOXP
    • Immunology
    •  
    • Adaptive Immunity
    •  
    • Regulatory T Cells
  • Function

    Probable transcription factor. Plays a critical role in the control of immune response.
  • Involvement in disease

    Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9BZS1The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • AIID
    • DIETER
    • Forkhead box P3
    • Forkhead box protein P3
    • FOXP3
    • FOXP3_HUMAN
    • FOXP3delta7
    • Immune dysregulation polyendocrinopathy enteropathy X linked
    • Immunodeficiency polyendocrinopathy enteropathy X linked
    • IPEX
    • JM2
    • MGC141961
    • MGC141963
    • OTTHUMP00000025832
    • OTTHUMP00000025833
    • OTTHUMP00000226737
    • PIDX
    • Scurfin
    • XPID
    hide
  • Database links

    • Entrez Gene: 50943 Human
    • Omim: 300292 Human
    • SwissProt: Q9BZS1 Human
    • Unigene: 247700 Human

Associated products

  • Alternative Versions

    • FITC Anti-5-methylcytosine (5-mC) antibody [5MC-CD] (ab179898)
    • Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] – Azide free (ab179914)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)

Protocols