Human IL-6 ELISA Kit High Sensitivity

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Key features and details

  • Sensitivity: 0.8 pg/ml
  • Range: 1.56 pg/ml – 50 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

Product name

Human IL-6 ELISA Kit High Sensitivity
See all IL-6 kits

Detection method

Colorimetric

Precision

Intra-assay
SamplenMeanSDCV%
Overall   4.4%
Inter-assay
SamplenMeanSDCV%
Sample 11825pg/ml312.2%
Sample 21812pg/ml18.7%
Sample 3186pg/ml113.7%
Sample 41841pg/ml25.9%
Sample 51831pg/ml14.9%

Sample type

Cell culture supernatant, Serum, Plasma

Assay type

Sandwich (quantitative)

Sensitivity

< 0.8 pg/ml

Range

1.56 pg/ml – 50 pg/ml

Recovery

93 %

Assay time

3h 45m

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Human

Product overview

Human IL-6 ELISA kit – High sensitivity is a sandwich ELISA kit for the quantitative measurement of IL-6 in supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognise both natural and recombinant Human IL-6.


A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples. 


This kit will recognize both endogenous and recombinant Human IL-6.


Get results in 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.

Platform

Microplate

Properties

Storage instructions

Store at +4°C. Please refer to protocols.
ComponentsIdentifier1 x 96 tests2 x 96 tests
10X Standard Diluent BufferBlack1 x 15ml1 x 25ml
200X Wash BufferWhite1 x 10ml2 x 10ml
Biotinylated Antibody DiluentRed1 x 7.5ml1 x 13ml
Biotinylated anti-IL-6Red1 x 0.4ml2 x 0.4ml
Chromogen TMB Substrate Solution 1 x 11ml1 x 24ml
HRP DiluentRed1 x 12ml1 x 23ml
IL-6 HS ControlYellow2 vials4 vials
IL-6 HS Standard (Lyophilized)Orange2 vials4 vials
IL-6 Microplate (12 x 8 well strips) 1 unit2 units
Plastic Plate Covers 1 x 2 units1 x 4 units
Standard Diluent (Serum) 1 x 7ml2 x 7ml
Stop ReagentBlack1 x 11ml2 x 11ml
Streptavidin-HRP 2 x 5µl4 x 5µl

Research areas

  • Immunology
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  • Innate Immunity
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  • Macrophage / Inflamm.
  • Immunology
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  • Innate Immunity
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  • Cytokines
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  • Interleukins
  • Neuroscience
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  • Neurology process
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  • Metabolism
  • Microbiology
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  • Organism
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  • Virus
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  • RNA Virus
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  • ssRNA positive strand virus
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  • SARS Coronavirus
  • Cancer
  •  
  • Invasion/microenvironment
  •  
  • Angiogenesis
  •  
  • Angiogenic growth factors
  • Cancer
  •  
  • Tumor immunology
  •  
  • Cytokines
  •  
  • Interleukins
  • Kits/ Lysates/ Other
  •  
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  • ELISA Kits
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  • ELISA Kits
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  • Cytokines and cytokine receptors ELISA kits
  • Metabolism
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  • Types of disease
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  • Obesity
  • Metabolism
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  • Types of disease
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  • Metabolic disorders
  • Neuroscience
  •  
  • Processes

Function

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

Involvement in disease

Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.

Sequence similarities

Belongs to the IL-6 superfamily.

Post-translational
modifications

N- and O-glycosylated.

Cellular localization

Secreted.

Protocols