Mouse and Rabbit Specific HRP/DAB IHC Detection Kit – Micro-polymer

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Overview

  • Product name

    Mouse and Rabbit Specific HRP/DAB IHC Detection Kit – Micro-polymer
  • Detection method

    Colorimetric
  • Assay type

    Micro-Polymer
  • Product overview

    This IHC kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.


    HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.


    This kit is an exact copy of EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit ab80436, except that it uses the HRP micro-polymer antibody used during the validation of Abcam’s antibodies in IHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining. 


    The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.

  • Notes

    The previous version of this kit, the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit, was used by researchers to stain over 150,000 slides since 2012 (based on volumes of reagent sold).

     

    Other IHC detection products

    For alternative kits, see anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469, and HRP-AEC micro-polymer IHC kits.

    For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components15 ml60 ml125 ml
    50x DAB Chromogen1 x 0.5ml1 x 2ml1 x 4ml
    DAB substrate1 x 15ml1 x 60ml1 x 125ml
    Goat anti-rabbit HRP Conjugate1 x 15ml1 x 60ml1 x 125ml
    Hydrogen Peroxide Block1 x 15ml1 x 60ml1 x 125ml
    Mouse Specifying Reagent (Complement)1 x 15ml1 x 60ml1 x 125ml
    Protein block1 x 15ml1 x 60ml1 x 125ml
  • Research areas

    • Kits/ Lysates/ Other
    •  
    • Kits
    •  
    • IHC Kits
  • Relevance

    HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.

Protocols

Available Options

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