Product nameMouse and Rabbit Specific HRP/DAB IHC Detection Kit – Micro-polymer
This IHC kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.
HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.
This kit is an exact copy of EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit ab80436, except that it uses the HRP micro-polymer antibody used during the validation of Abcam’s antibodies in IHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining.
The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.
The previous version of this kit, the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit, was used by researchers to stain over 150,000 slides since 2012 (based on volumes of reagent sold).
Other IHC detection products
For alternative kits, see anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469, and HRP-AEC micro-polymer IHC kits.
For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 15 ml 60 ml 125 ml 50x DAB Chromogen 1 x 0.5ml 1 x 2ml 1 x 4ml DAB substrate 1 x 15ml 1 x 60ml 1 x 125ml Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 60ml 1 x 125ml Hydrogen Peroxide Block 1 x 15ml 1 x 60ml 1 x 125ml Mouse Specifying Reagent (Complement) 1 x 15ml 1 x 60ml 1 x 125ml Protein block 1 x 15ml 1 x 60ml 1 x 125ml
- Kits/ Lysates/ Other
- IHC Kits
RelevanceHRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.