AF/LE Purified Anti-Human CD20 Antibody[2H7]

Test Application

Verified Samples

Reactivity Application

Human FCM

( peripheral blood lymphocytes,peripheral blood lymphocytes )
Dilution

FCM 0.2 μg(1×10^5-5×10^5 cells)

Product Details

Clonality	Monoclonal
Isotype	Mouse IgG2b, κ
Concentration	1mg/mL
Storage	Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Buffer	Sterile PBS. < 1.0 EU per mg of the antibody as determined by the LAL method
Purification Method	>98%, Protein A/G purified
Research Areas	Costimulatory Molecules; Immunology
Clone No.	2H7
Conjugation	Unconjugated

Immunogen Details

Immunogen	Recombinant Human CD20 protein
Abbre	CD20
Synonyms	B-lymphocyte surface antigen B1;Bp35;Leukocyte surface antigen Leu-16;MS4A1
Swissprot	P11836

Related Products

Cat. No.	Product Name	Sizes
E-AB-F1212A	Purified Anti-Human CD20 Antibody[2H7]	50μg / 500μg / 1mg
E-AB-F1212C	FITC Anti-Human CD20 Antibody[2H7]	50μg / 500μg / 1mg
E-AB-F1212D	PE Anti-Human CD20 Antibody[2H7]	50μg / 500μg / 1mg

Background

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.