MTT Cell Proliferation and Cytotoxicity Assay Kit

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Detection principle

MTT is 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide which can be reduced by some dehydrogenases in mitochondria to form a crystalline dark purple product formazan. Formazan can be completely dissolved by DMSO and its absorbance is near 570 nm wavelength. The more and faster the cells proliferate, the darker the color is, and the more cytotoxic, the lighter the color is. For the same cells, there is a linear relationship between the depth darkness of color and the number of cells.

General Information

Application Viability/Proliferation/Cytotoxicity
Detection method Colorimetric Method
Sample type Cell samples
Detection instrument Microplate reader
Other reagents required DMSO
Storage 2~8°C, shading light
Shipping Ice bag
Expiration date 6 months