Mouse Crh(Corticoliberin) ELISA Kit

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Product Name Mouse Crh(Corticoliberin) ELISA Kit
Catalogue No. EM0465
Alias Crh ELISA Kit, Corticotropin Releasing Hormone ELISA Kit, Corticoliberin ELISA Kit, Corticotropin-releasing factor ELISA Kit, CRF ELISA Kit, Corticotropin-releasing hormone ELISA Kit
Detection Method Competitive ELISA, Coated with Antigen
Application Crh ELISA Kit allows for the in vitro quantitative determination of Crh concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size 96T
Range 0.313-20ng/ml
Sensitivity < 0.188ng/ml
Species

UniProt ID

Mouse

Q8CIT0

Standard Curve
Storage 2-8 ℃ for 6 months
Recovery Matrices listed below were spiked with certain level of Crh and the recovery rates were calculated by comparing the measured value to the expected amount of Crh in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-99 92
EDTA plasma(n=5) 86-102 93
heparin plasma(n=5) 89-103 96
Linearity The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Crh and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8
serum(n=5) 85-99% 85-99% 87-105%
EDTA plasma(n=5) 90-99% 89-92% 85-98%
heparin plasma(n=5) 80-100% 83-99% 80-97%
CV(%) Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note For Research Use Only

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