TUNEL In Situ Apoptosis Kit (HRP-DAB Method)

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Detection principle

When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3′-OH can be added with a biotinylated dUTP (Biotin-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), followed by the binding of horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP). Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis.

General Information

Application DNA Fragmentation
Detection method HRP-DAB
Sample type Paraffin section, frozen section, cell slide
Assay time 3 hours
Detection instrument Light microscope
Other reagents required 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),3% H2O2(E-IR-R115),Xylene,Ethanol,DNase I,PBS(E-IR-R187),ddH2O,Hematoxylin(E-IR-R120),Neutral gum(E-IR-R118)
Storage -20°C, shading light. Refer to the Manual.
Shipping Ice bag
Expiration date 12 months