Malondialdehyde (MDA) Fluorometric Assay Kit

Cat.No.:E-BC-F007
Detection instrument: Fluorescence microplate reader (Ex/Em=520 nm/550 nm)

Detection method: Fluorescence method

Detection principle

MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which is proportional to the fluorescence intensity at the excitation wavelength of 520 nm and emission wavelength of 550 nm.

Performance characteristics

Synonyms	MDA
Sample type	serum, plasma, animal tissue, and cell
Sensitivity	0.04 μmol/L
Detection range	0.04-10 μmol/L
Detection method	Fluorescence method
Assay type	Quantitative
Precision	Average inter-assay CV: 4.5-7.0%Average intra-assay CV: 2.3-4.3%
Other instruments required	Micropipettor, Water bath, Vortex mixer, Centrifuge
Storage	2-8℃
Valid period	12 months