Glucose (GLU) Fluorometric Assay Kit

Cat.No.:E-BC-F037
Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/590 nm)

Detection method: Fluorescence method

Detection principle

Glucose oxidase can catalyze the oxidation of glucose into gluconic acid and produce hydrogen peroxide. In the presence of peroxidase, hydrogen peroxide reacts with the non-fluorescent substance to form fluorescent substance. The glucose content can be calculated indirectly by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Performance characteristics

Synonyms	GLU
Sample type	Serum, plasma, urine、saliva、milk、cell
Sensitivity	0.1 μmol/L
Detection range	0.1-20 μmol/L
Detection method	Fluorescence method
Assay type	Quantitative
Assay time	25 min
Precision	Average inter-assay CV: 2.800%Average intra-assay CV: 1.700%
Other instruments required	Pipettor, Water bath, Centrifuge
Storage	-20℃
Valid period	12 months

Blood glucose level was significantly increased in STZ group (***P<0.001) and restored in treated group (**P<0.01).

J Liu et al investigate the function of circ-ITCH for improving renal inflammation and fibrosis in streptozotocin-induced mice. Glucose (GLU) concentration in mouse blood was determined using glucose fluorometric assay kit (E-BC-F037).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.1-20 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):