Cell and tissue samples are treated with lysates containing protease inhibitors and phosphatase inhibitors to prevent the enzymes in the sample from being released to hydrolyze or dephpsphprylate protein due to the disruption of the membrane system.
|Other instruments required
|High-speed freezing centrifuge, 5mL Glass homogenizer
|Other reagents required
|Double distilled water, PBS(0.01 M，pH=7.4)
|Reagent 1: 2-8℃; Others: -20℃