Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

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Product Name Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
Catalogue No. EM0183
Alias TNFα ELISA Kit, Tumor Necrosis Factor Alpha ELISA Kit, TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Detection Method Sandwich ELISA, Double Antibody
Application TNF-α ELISA Kit allows for the in vitro quantitative determination of TNFα concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size 96T
Range 3.906-250pg/ml
Sensitivity 3.906-250pg/ml

UniProt ID



Standard Curve
Storage 2-8 for 6 months
Recovery Matrices listed below were spiked with certain level of TNFα and the recovery rates were calculated by comparing the measured value to the expected amount of TNFα in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-105 96
EDTA plasma(n=5) 85-105 96
heparin plasma(n=5) 85-105 91
Linearity The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8
serum(n=5) 90-105% 86-100% 93-105%
EDTA plasma(n=5) 84-99% 96-100% 82-100%
heparin plasma(n=5) 81-100% 82-98% 81-100%
CV(%) Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note For Research Use Only