AzureCyto In-Cell Western Kit

The AzureCyto In-Cell Western Kit contains all reagents necessary for staining of whole cells for total cell normalization and simultaneous detection of two biomarkers. The AzureCyto Permeabilization Solution removes membrane lipids and facilitates permeabilization of the nuclear membrane to allow the penetration of antibodies into cells for the detection of intracellular and nuclear proteins.

Can be co-incubated with primary antibodies to streamline the detection process and reduce the number of assay steps. Each kit includes secondary antibodies conjugated to AzureSpectra 700 and AzureSpectra 800 to enable the simultaneous detection of two biomarkers. Includes all reagents necessary to perform five 96-well in-cell Western assays.

Includes:

• AzureCyto Permeabilization Solution: removes membrane lipids and facilitates permeabilization of the nuclear membrane to allow the penetration of antibodies into cells for the detection of intracellular and nuclear proteins
• AzureCyto Block Solution: Blocking solution formulated for cell-based assays and fluorescence detection
• AzureCyto Total Cell Stain: A sensitive and linear stain that can be co-incubated with primary antibodies to streamline the detection process and reduce the number of assay steps
• AzureSpectra Secondary Antibodies: For simultaneous detection of two biomarkers