10x Ammonium Buffer

Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg²⁺ concentrations or the annealing temperatures.

FEATURES

• Promotes amplification on most DNA templates
• High yield
• High specificity
• Tolerance for primer annealing temperature
• 10x Buffer

AVAILABLE IN 4 FORMULATIONS:

• 15 mM MgCl_2,
• Mg²⁺ free
• Tween free
• Mg²⁺ free + Tween free

DESCRIPTION

Ammonium Buffer promotes high specificity over a broad range of annealing temperatures and Mg²⁺ concentrations. Ammonium Buffer also works well when dealing with difficult templates, e.g. GC-rich DNA sequences.

TIP – CHOOSE THE RIGHT BUFFER

Mg²⁺ free buffer is recommended if you need to optimise Mg²⁺ concentrations in your PCR set-up, especially if your application requires Mg²⁺ concentration lower than 1.5 mM.

Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.

PERFORMANCE OF AMPLIQON PCR BUFFER SYSTEM

Ammonium Buffer: Minimal need for optimisation.
A broad range of Mg²⁺ concentrations and temperatures results in specific products with high yield (Lanes 1.5 – 2.5 and lanes 57-66)

Standard Buffer: Optimisation is needed.
A narrow range of Mg²⁺ concentrations and temperatures results in specific products. (Lanes 1.5 – 2.0 and lanes 60-66)

Combination Buffer: Optimisation is needed.
A narrow range of Mg²⁺ concentrations and temperatures results in specific products with high yields. (Lane 1.5 and lanes 60-66)

Examples of PCR amplification of ENG9. TEMPase and the indicated buffers were used at the indicated Mg²⁺ concentrations or temperatures. The first part shows a Mg²⁺ dilution series from 0.5 – 4.5 mM MgCl₂, at 60°C. The second part shows a temperature gradient from 51 – 66 °C at 1.5 mM MgCl₂. M: marker.