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PCR Grade Water

5x PCR Buffer RED

Delivery date subjected to stock availability.

Kindly contact us to confirm stock available before placing the order.

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5x PCR Buffer RED is a ready to use PCR buffer, including all components for standard PCR applications.

FEATURES

  • All in one Buffer for highest convenience
  • Direct gel loading onto agarose gels
  • Red colour enables easy visualisation of pipetting and loading
  • Dye front runs at 1000 – 2000 bp on DNA 0.5 – 1.5 % agarose
  • Same specifications as Ammonium Buffer
  • 5x Buffer

DESCRIPTION

5x PCR Buffer RED offers the same specification as Ammonium Buffer.  It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures. The included red dye and density reagent, eliminates the need for loading dye as well as the time consuming sample preparation before electrophoresis.

 

DIRECT GEL LOADING

After end of amplification PCR products can be loaded directly onto agarose and SDS DNA gels. The red dye provides easy visualisation of both pipetting and gel loading. The red dye front runs at 1000 – 2000 bp on 0.5 – 1.5 % agarose gels.

 

5X PCR BUFFER RED PROMOTES SPECIFIC AND CLEAR RESULTS

5x PCR Buffer RED promotes specific and clear results. The same DNA target was evaluated using the same PCR set-up in duplicates amplified by either Ampliqon Taq DNA Polymerase or TEMPase Hot Start DNA Polymerase.