Taq DNA Polymerase

Ampliqon Taq DNA Polymerase 5 U/µl is ideal for routine PCR applications requiring high yield and also reliable DNA amplification. Taq DNA Polymerase 5 U/µl is available in different sizes and buffer combinations.

FEATURES

A thermostable recombinant DNA Polymerase
High product yield
dUTP incorporation possible
processes up to 5 kb
Leaves a 3’dA overhang

DESCRIPTION

Ampliqon Taq DNA Polymerase has a molecular weight of 95 kDa and exhibits both a 5’→3′ DNA polymerase and a 5’→3′ exonuclease activity. The 5’→3′ exonuclease activity leaves a 3’dA overhang on the PCR product, which are convenient for direct T-A cloning. Taq DNA Polymerase lacks 3’→5′ exonuclease activity and proofreading ability.

Taq DNA polymerase is suitable for standard testing, routine PCR, screening and high throughput testing.

Taq DNA polymerase also exits in glycerol format for automation and lyophilization: Taq DNA Polymerase Glycerol Free

COMPARISON BETWEEN AMPLIQON TAQ DNA POLYMERASE AND A COMPETITOR

Ampliqon Taq DNA Polymerase was compared with a Taq DNA Polymerase from a well-recognised competitor. Three different DNA target varying in length PAH (203 bp), ENG9 (293 bp) and ENG 5 (480 bp) were evaluated. Ampliqon Taq DNA Polymerase performed equally well or better in this study. Furthermore, Ampliqon Taq DNA Polymerase displays a higher specificity on the PAH and ENG5 targets, than the competing Taq DNA Polymerase. Each Amplification has been conducted according to supplier’s manual.