TEMPase Hot Start DNA Polymerase Glycerol Free 5 U/µl is a glycerol free formulation of our regular TEMPase Hot Start DNA Polymerase 5 U/µl and is well suited for automated high throughput testing, freeze drying or where accurate pipetting of small volumes are crucial
- Convenient reaction set up at room temperature
- Automated high throughput testing
- Freeze drying
- dUTP incorporation possible
- Increased sensitivity, specificity and product yield
TEMPase Hot Start DNA Polymerase Glycerol Free 5 U/µl is a chemically modified version of Ampliqon Taq DNA Polymerase and is activated by heat treatment.
A chemical moiety is attached to the enzyme at the active site, which renders the TEMPase Hot Start enzyme inactive at room temperature.
During set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. Once the PCR reaction reaches the optimal activation temperature, the chemical moiety is cleaved releasing the active TEMPase Hot Start DNA Polymerase, resulting in higher specificity, sensitivity and yield compared to standard Ampliqon Taq DNA polymerase.
Glycerol is normally a major part of the storage buffer of enzymes and acts as a cryoprotectant. Glycerol disrupts the water structure and makes the buffer more cell like, hence stabilising the polymerase. Glycerol is a highly viscous liquid and is therefore difficult and time-consuming to pipet accurately, especially in smaller volumes. As a consequence, pipetting glycerol in fast, robot-aided automation processes is almost an unsolvable challenge. Furthermore, the presence of glycerol in the enzyme buffer makes freeze drying impossible.
GLYCEROL FREE FOR AUTOMATION AND FREEZE DRYING