Phosphate buffered saline

tablet

Synonym(s):PBS

Properties

form

tablet

Quality Level

200

pH

7.2-7.6 (1?tablet/200 mL)

solubility

water: soluble

application(s)

diagnostic assay manufacturing

Description

General description

Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.

Application

Phosphate buffered saline has been used-

• for the production of platinum nanoparticles (NP-Pt) hydrocolloids
• for the washing of blood sample and SEM (scanning electron microscope) stubs prior to SEM
• for preparing the solution of fluorescent lectin wheat germ agglutinin (WGA)
• for the dilution of capture antibody during ELISA (enzyme linked immunosorbent assay)
• for suspension of cell pellets during centrifugation
• in immunoassay procedures

Packaging

50, 100 tablets in poly bottle

Reconstitution

One tablet dissolved in 200 mL of deionized water yields 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25??C.

Safety Information

Storage Class Code

13 – Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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