Lyticase from Arthrobacter luteus

free of DNA contaminants, suitable for Microbiome research, ?2000?units/mg protein, lyophilized powder

Synonym(s):

(1,3)-?-D-Glucan endohydrolase, 1,3-?-Glucan glucohydrolase

Properties

biological source

bacterial (Arthrobacter luteus)

form

lyophilized powder

specific activity

?2000?units/mg protein

feature

DNA free

shipped in

dry ice

storage temp.

?20?C

Description

General description

Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.

Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.

Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.