Dulbecco′s Modified Eagle′s Medium – high glucose

SKU: N/A Categories: ,

With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

DME, DMEM

Properties

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: high
sodium pyruvate: yes
NaHCO3: no
HEPES: no
phenol red: yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

Description

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.

Quantity

Formulated to contain 13.5 grams of powder per liter of medium.

Reconstitution

Supplement with 3.7 g/L sodium bicarbonate

Safety Information

Storage Class Code

13 – Non Combustible Solids

WGK

WGK 2