Blue-ray EzQuant Quantification Assay Kits

EzQuant quantification assays are your trusted choice for dsDNA HS, ssDNA, and RNA HS detection. These assays utilize a highly specific fluorescent dye that binds precisely to the target molecule. This allows accurate quantification down to 10 pg/µl, even in the presence of contaminants or degraded DNA/RNA. EzQuant quantification assays only require a 1 µl sample and a quick 2-minute incubation, to significantly enhance the efficiency of your quantification processes.

EzQuant Quantification Assay Kits

EzQuant Quantification Assays are your trusted choice for dsDNA HS, ssDNA, and RNA HS detection. These assays utilize a highly specific fluorescent dye that binds precisely to the target molecule. This allows accurate quantification down to 10 pg/µl, even in the presence of contaminants or degraded DNA/RNA. EzQuant Quantification Assays only require a 1 µl sample and a quick 2-minute incubation. This significantly enhances the efficiency of your quantification processes.

Features and Benefits

Samples can be as little as 1 µl.
Only 2 minutes of incubation is needed.
Results can be read in seconds.

Figure 1. The initial sample concentration for EzQuant Quantification Assays and UV absorbance measurements ranges from 1 – 20 µl. UV absorbance measurement can quantify high concentration samples, but are not selective for DNA or RNA.

EzQuant 1X dsDNA HS Quantification Kit

EzQuant 1X dsDNA HS Quantification Kit contains a highly specific fluorescent dye designed for samples with low dsDNA concentration and which also has good tolerance to contaminants. Ready-to-use working solutions and standards are provided. Easy and accurate DNA sample quantification, within a range of 10 pg/μl to 100 ng/μl, can be performed with EzCube Fluorometer for samples with volumes from 1 – 20 µl.

Sensitivity

EzQuant 1X dsDNA HS Quantification Assay has higher sensitivity to dsDNA than RNA.

Figure 2. The DNA sensitivity of EzQuant 1X dsDNA HS Quantification Assay was determined from an assay of 10 µl samples containing different concentrations of DNA, RNA, and a mixture of DNA and RNA.

Linearity

Figure 3. Linearity between measured and prepared dsDNA concentrations for EzQuant and Qubit™ assays. Samples (10 µl) of two standard solutions, S1 (0 ng/µl) and S2 (10 ng/µl) were used to establish a standard curve, and dilutions of S2 were used for quantification.