Nuclear Fast Red

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for microscopy (Bot., Hist.)

Synonym(s):
4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt

Properties

grade

for microscopy (Bot., Hist.)

Quality Level

form

powder

solubility

methanol: water (1:1): 0.01 g/10 mL, red

εmax

≥275 at 550-560 nm in DMSO
≥300 at 516-526 nm in DMSO

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Nc1c(O)c(c(O)c2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O

InChI

1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1

InChI key

IFSXZLJQEKGQAF-UHFFFAOYSA-M

Description

General description

Nuclear Fast Red (C.l. 60760) is a substituted hydroxy anthraquinone chromophore that can chelate metal ions. It is also known as kernechtrot and 4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt.

Application

Nuclear fast red has been used for the nuclear staining of PB (Prussian blue) positive cells in the rostral sub-epidermis and upper beak coronal region isolated from pigeons.Recent applications include:
  • staining sperm and spermatids in semen
  • identifying areas for PCR analysis in paraffin-embedded tissues
  • nuclear counterstaining following immunostaining and silver staining
Used as a biological stain

Biochem/physiol Actions

Nuclear fast red dye has anthraquinonic nature and is used either alone or in combination with other dyes. It is mainly used for staining of nuclei.

Safety Information

Storage Class Code

11 – Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable