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Deoxyribonucleic acid from calf thymus
Deoxyribonucleic acid from calf thymus
Type XV, Activated, lyophilized powder
Synonym(s):
Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
Properties
grade
for molecular biology
Quality Level
200
type
Type XV
form
lyophilized powder
storage temp.
−20°C
Description
General description
High quality double-stranded template DNA isolated from the thymus of male and female calves.
Application
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Packaging
1, 5 mg in glass bottle
Features and Benefits
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
• High quality template DNA
Quantity
One mg DNA is approx. 20 A260 units.
Preparation Note
Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.
Reconstitution
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
Safety Information
Storage Class Code
12 – Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
