Fetal Bovine Serum

non-USA origin, sterile-filtered, suitable for cell culture

Synonym(s):

Fetal Calf sera, Fetal Calf serum, FBS, FCS, sera, serum

Properties

biological source

bovine fetus

Quality Level

400

sterility

sterile-filtered

product line

FBS Classic

composition

hemoglobin, ≤25 mg/dL

origin

non-USA origin

availability

not available in Canada, USA

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

Description

Application

FBS is used in a broad range of cell culture applications.

Biochem/physiol Actions

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems. It provides many non-defined growth promoting and survival enhancing factor to cells in culture. Derived from clotted blood, it is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.