Anti-HLA Class I antibody [MEM-147]

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Key features and details

  • Mouse monoclonal [MEM-147] to HLA Class I
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG1

Overview

Product name

Anti-HLA Class I antibody [MEM-147]
See all HLA Class I primary antibodies

Description

Mouse monoclonal [MEM-147] to HLA Class I

Host species

Mouse

Specificity

Reacts with all human classical MHC Class I molecules in native cell-surface forms. Positive in immunoblotting with many (not all) MHC Class I allelic forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). Positive in Western blotting (non-reducing conditions) with most HLA-B and HLA-C molecules, but not HLA-A. Epitope specificity is very similar to classical antibody W6/32 (ab7855).

Tested applications

Suitable for: Flow CytWBmore details

Species reactivity

Reacts with: Human

Immunogen

Tissue, cells or virus corresponding to HLA Class I. PHA-activated peripheral blood lymphocytes

General notes

 

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

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Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Storage buffer

pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS

Concentration

  • 100 µg at 1 mg/ml

Purity

Protein A purified

Purification notes

Purified from TCS. Purity >95% by SDS-PAGE.

Clonality

Monoclonal

Clone number

MEM-147

Isotype

IgG1

Research areas

  • Immunology
  •  
  • Adaptive Immunity
  •  
  • MHC
  •  
  • Class I

Protocols