Oxidized LDL Assay Kit (MDA-LDL, Human)

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Key features and details

  • Detection method: Colorimetric
  • Platform: Microplate
  • Sample type: Plasma, Serum
  • Sensitivity: 50 ng/ml

Overview

Product name

Oxidized LDL Assay Kit (MDA-LDL, Human)
See all oxidized phospholipids kits

Detection method

Colorimetric

Sample type

Serum, Plasma

Sensitivity

50 ng/ml

Species reactivity

Reacts with: Human

Product overview

Oxidized LDL Assay Kit (MDA-LDL, Human) ELISA Kit (ab242302) is developed for the detection and quantitation of oxidized phospholipids (OxPL) associated with human LDL in plasma, serum or other biological fluid samples.


The kit contains an OxLDL Standard and has a detection sensitivity limit of <15 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.

Tested applications

Suitable for: ELISA

Platform

Microplate

Properties

Storage instructions

Please refer to protocols.
Components1 x 96 tests
10X Wash Buffer1 x 100ml
Anti-MDA Antibody Coated Plate1 unit
Assay Diluent1 x 50ml
Biotinylated Anti-Human ApoB-100 Antibody (1000X)1 x 20µl
Blocking Reagent (100X)1 x 200µl
LDL Precipitation Solution (2X)1 x 20ml
OxLDL Standard1 x 25µl
Stop Solution1 x 12ml
Streptavidin-Enzyme Conjugate1 x 20µl
Substrate Solution1 x 12ml

Relevance

Lipoproteins are submicroscopic particles composed of lipid and protein held together by noncovalent forces. Their general structure is that of a putative spheroidal microemulsion formed from an outer layer of phospholipids, unesterified cholesterol, and proteins, with a core of neutral lipids, predominately cholesteryl esters and triacylglycerols (TAG). Low density lipoprotein (LDL) is the major transport protein for cholesterol in human plasma. LDL is a spherical particle with a diameter of 20-25 nm. Each LDL particle contains cholesteryl esters in its core which are surrounded by a hydrophilic coat composed of phospholipids, cholesterol, and one molecule of a hydrophobic protein known as apolipoprotein B-100. LDL cholesterol, sometimes referred to as “bad” cholesterol, is even more dangerous when it becomes oxidized. Oxidized LDL (OxLDL) is more reactive with surrounding tissues and can collect within the inner-lining of arteries. Macrophages, cholesterol, and other lipids can accumulate at the site (atherosclerosis), ultimately forming a plaque that can lead to heart attack, stroke or death. LDL oxidation affects both the protein and phospholipids of LDL. Reactive aldehyde products formed during the oxidation of polyunsaturated fatty acids, such as malondialdehyde (MDA) and 4hydroxynonenal (HNE), are capable of attaching covalently to the e-amino groups of lysine residues of of ApoB-100 to form MDA-Lys and HNE-Lys adducts (MDA-LDL and HNE-LDL). Advanced glycosylation, such as the formation of CML-LDL and CEL-LDL, are also involved in LDL oxidation.

Protocols