Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric)

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Key features and details

  • Assay type: Quantitative
  • Detection method: Colorimetric/Fluorometric
  • Platform: Microplate reader
  • Assay time: 1 hr 20 min
  • Sample type: Cell culture extracts, Plasma, Tissue Extracts, Urine
  • Sensitivity: 0.1 nmol/well

Overview

Product name

Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric)
See all Lipid Peroxidation kits

Detection method

Colorimetric/Fluorometric

Sample type

Urine, Plasma, Cell culture extracts, Tissue Extracts

Assay type

Quantitative

Sensitivity

> 0.1 nmol/well

Assay time

1h 20m

Product overview

Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970) provides a convenient tool for sensitive detection of malondialdehyde (MDA).


In the lipid peroxidation assay protocol, the MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.

Properties

Storage instructions

Store at -20°C. Please refer to protocols.
ComponentsIdentifier100 tests2000 tests
BHTPurple1 x 1ml20 x 1ml
MDA Lysis BufferWM1 x 25ml20 x 25ml
MDA Standard (4.17M)Yellow1 x 100µl20 x 100µl
Phosphotungstic Acid SolutionNM1 x 12.5ml20 x 12.5ml
TBANM4 vials80 vials

Research areas

  • Signal Transduction
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  • Metabolism
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  • Lipid metabolism
  • Kits/ Lysates/ Other
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  • Kits
  •  
  • Cell Metabolism Kits
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  • Lipid Metabolism Kits
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  • Obesity
  • Kits/ Lysates/ Other
  •  
  • Kits
  •  
  • Cell Damage Kits
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  • Oxidative stress
  • Kits/ Lysates/ Other
  •  
  • Kits
  •  
  • Cell Metabolism Kits
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  • Lipid Metabolism Kits
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  • Lipid metabolism
  • Metabolism
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  • Pathways and Processes
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  • Redox metabolism
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  • Fatty acid oxidation
  • Kits/ Lysates/ Other
  •  
  • Kits
  •  
  • Cell Damage Kits
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  • Lipid oxidation

Relevance

Lipid peroxidation refers to the oxidative degradation of lipids and is a well-defined mechanism of cellular damage. The formation of lipid peroxidation products leads to spread of free radical reactions leading to cell damage.

Alternative names

  • TBAR
  • TBARS

Protocols