ATP Assay Kit (Colorimetric/Fluorometric)

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Key features and details

  • Assay type: Quantitative
  • Detection method: Colorimetric/Fluorometric
  • Platform: Microplate reader
  • Assay time: 1 hr
  • Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
  • Sensitivity: 1 µM

Overview

Product name

ATP Assay Kit (Colorimetric/Fluorometric)

Detection method

Colorimetric/Fluorometric

Sample type

Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate

Assay type

Quantitative

Sensitivity

< 1 µM

Assay time

1h 00m

Product overview

ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) use a robust, simple method; the ATP assay protocol relies on the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (ODmax = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.


This kit can detect as low as 1 µM of ATP in various samples.


ATP assay protocol summary:
– add samples (deproteinized) and standards to wells
– add reaction mix and incubate for 30 min at room temp
– analyze with microplate reader


Chinese protocol available. See protocols section below.


If you require a more sensitive product, we recommend Luminescent ATP Detection Assay Kit (ab113849), which can detect as low as 1 pM of ATP.

Notes

This product is manufactured by BioVision, an Abcam company and was previously called K354 ATP Colorimetric/Fluorometric Assay Kit. K354-100 is the same size as the 100 test size of ab83355.

Related assays

Review the cell health assay guide to learn about kits to perform a cell viability assay, cytotoxicity assay and cell proliferation assay. 

Review the metabolism assay guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

How other researchers have used ATP Assay Kit ab83355

This ATP assay kit has been used in publications in a variety of sample types, including:
– Human: cell culture lysates1, primary monocyte cell culture lysates2, HCT116 cell culture supernatants3
– Mouse: heart tissue4, liver5, C2C12 and L929 cell lysates6, primary thymocyte cell culture lysates7, cardiac tissue8
– Rat: primary hippocampal neuron cell culture lysates9, liver tissue10, skeletal muscle11
– Pig: kidney cell culture lysates12, heart tissue13 
– C elegans tissue14
– Chlamydomonas reinhardtii algae15

Platform

Microplate reader

Properties

Storage instructions

Store at -20°C. Please refer to protocols.
ComponentsIdentifier100 tests2000 tests
ATP Assay BufferWM1 x 25ml20 x 25ml
ATP Converter (lyophilized)Blue1 vial20 vials
ATP Probe (in DMSO)Red1 x 200µl20 x 200µl
ATP Standard (1 µmol; lyophilized)Yellow1 vial20 vials
Developer Mix (lyophilized)Green1 vial20 vials

Research areas

  • Kits/ Lysates/ Other
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  • Kits
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  • Cell Metabolism Kits
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  • Other Metabolism Assay
  • Kits/ Lysates/ Other
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  • Cell Metabolism Kits
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  • Cell Viability and Senescence Kits
  • Kits/ Lysates/ Other
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  • Kits
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  • Cell Metabolism Kits
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  • Intermediary Metabolism Kits
  • Kits/ Lysates/ Other
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  • Kits
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  • Cell Signaling Kits
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  • Phosphatase assay kits
  • Kits/ Lysates/ Other
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  • Kits
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  • Cell Damage Kits
  •  
  • Cell Damage

Alternative names

  • Adenosine 5′ triphosphate

Protocols