Mouse Osteopontin ELISA Kit

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Key features and details

  • Sensitivity: 4 pg/ml
  • Range: 2.74 pg/ml – 2000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

Product name

Mouse Osteopontin ELISA Kit
See all Osteopontin kits

Detection method

Colorimetric

Sample type

Cell culture supernatant, Serum, Plasma

Assay type

Sandwich (quantitative)

Sensitivity

< 4 pg/ml

Range

2.74 pg/ml – 2000 pg/ml

Recovery

100 %

Sample specific recovery
Sample typeAverage %Range
Cell culture supernatant85.4678% – 105%
Serum115.6103% – 122%
Plasma110.487% – 130%

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Mouse

Product overview

Abcam’s Osteopontin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Osteopontin in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Osteopontin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Osteopontin antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Platform

Microplate

Properties

Storage instructions

Store at -20°C. Please refer to protocols.
Components1 x 96 tests
20X Wash Buffer1 x 25ml
400X HRP-Streptavidin Concentrate1 x 200µl
5X Assay Diluent B1 x 15ml
Assay Diluent C1 x 30ml
Biotinylated anti-Mouse Osteopontin2 vials
Osteopontin Microplate (12 x 8 wells)1 unit
Recombinant Mouse Osteopontin Standard (lyophilized)2 vials
Stop Solution1 x 8ml
TMB One-Step Substrate Reagent1 x 12ml

Research areas

  • Cardiovascular
  •  
  • Angiogenesis
  •  
  • Adhesion / ECM
  •  
  • Extracellular Matrix
  • Signal Transduction
  •  
  • Cytoskeleton / ECM
  •  
  • Extracellular Matrix
  •  
  • ECM Proteins
  •  
  • Osteopontin
  • Signal Transduction
  •  
  • Cytoskeleton / ECM
  •  
  • Extracellular Matrix
  •  
  • Structures
  •  
  • Bone
  • Signal Transduction
  •  
  • Metabolism
  •  
  • Vitamins / Minerals
  • Stem Cells
  •  
  • Mesenchymal Stem Cells
  •  
  • Osteogenesis
  • Cancer
  •  
  • Invasion/microenvironment
  •  
  • ECM
  •  
  • Extracellular matrix
  •  
  • Other
  • Cancer
  •  
  • Tumor biomarkers
  •  
  • Other
  • Developmental Biology
  •  
  • Organogenesis
  •  
  • Skeletal development
  •  
  • Bone
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Function

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Tissue specificity

Bone. Found in plasma.

Sequence similarities

Belongs to the osteopontin family.

Post-translational
modifications

Extensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium.

Cellular localization

Secreted.

Protocols