Human IL-7 ELISA Kit

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Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 5.9 pg/ml
  • Range: 18.8 pg/ml – 1200 pg/ml
  • Sample type: Cell culture media, Cit plasma, EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

Product name

Human IL-7 ELISA Kit

Detection method

Colorimetric

Precision

Intra-assay
SamplenMeanSDCV%
serum8  4.4%
Inter-assay
SamplenMeanSDCV%
serum3  2.1%

Sample type

Serum, Cell culture media, EDTA Plasma, Cit plasma

Assay type

Sandwich (quantitative)

Sensitivity

5.9 pg/ml

Range

18.8 pg/ml – 1200 pg/ml

Recovery

 

Sample specific recovery
Sample typeAverage %Range
Serum8078% – 84%
Cell culture media10196% – 104%
EDTA Plasma8179% – 82%
Cit plasma8479% – 92%

Assay time

1h 30m

Assay duration

One step assay

Species reactivity

Reacts with: Human

Product overview

Human IL-7 ELISA Kit (ab270218) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-7 protein in edta plasma, serum, cit plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-7 with 5.9 pg/ml sensitivity.


SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


        – Single-wash protocol reduces assay time to 90 minutes or less
        – High sensitivity, specificity and reproducibility from superior antibodies
        – Fully validated in biological samples
        – 96-wells plate breakable into 12 x 8 wells strips


A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Notes

IL-7 (Interleukin 7) is a 20kDa hematopoietic growth factor secreted by stromal cells residing in the bone marrow, thymus, spleen and gut. IL-7 plays a critical role during early T-cell development of naïve and memory CD8+ T cells, as well as during the proliferation and differentiation of B cells. Furthermore, IL-7 has been shown to play a key role in coreceptor reversal, a process which induces the expression of CD8 receptor in a T-cell that has begun differentiation as a CD4+ T cell. IL-7 belongs to a family of short-chain four-helical bundle cytokines. These family members signal through heterodimeric receptor complexes that contain the common cytokine receptor gamma-chain, and activate signaling pathways that promote cell proliferation and survival such as the PI3K, MAPK, and JAK/STAT. IL-7 has been implicated in the development of rheumatoid arthritis (RA), HIV, acute graft-vs-host disease (aGVHD) and X-linked severe combined immunodeficiency (XSCID). In RA, IL-7 activates the production of TNF-alpha by CD4+ T cells and macrophages within the synovial fluid, leading to the inflammation characteristic of RA. In patients with HIV, IL-7 homeostasis is impaired due to decreased expression of the IL-7R.  The loss of T-cell homeostasis induces the lymphopenic condition characteristic of an HIV infection. Furthermore, the T-cell cytotoxic regimen prior to bone marrow transplantation induces IL-7 secretion, stimulating growth of residual allo-reactive T cells. These allo-reactive T-cells recognize and attack the transplanted cells and host tissues which lead to the development of aGVHD. Additionally, IL-7 knockout studies in mice have demonstrated that IL-7 is crucial for T cell survival as absence of IL-7 leads to XSCID. The rodent IL-7 proteins have low homology to human IL-7; only 56-57% amino acids sequence identity.  

Platform

Pre-coated microplate (12 x 8 well strips)

Properties

Storage instructions

Store at +4°C. Please refer to protocols.
Components1 x 96 tests
10X Human IL-7 Capture Antibody1 x 600µl
10X Human IL-7 Detector Antibody1 x 600µl
10X Wash Buffer PT (ab206977)1 x 20ml
Antibody Diluent 4BI1 x 6ml
Human IL-7 Lyophilized Recombinant Protein2 vials
Plate Seals1 unit
Sample Diluent NS (ab193972)1 x 50ml
SimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unit
Stop Solution1 x 12ml
TMB Development Solution1 x 12ml

Research areas

  • Immunology
  •  
  • Innate Immunity
  •  
  • Cytokines
  •  
  • Interleukins
  • Neuroscience
  •  
  • Processes

Function

Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.

Sequence similarities

Belongs to the IL-7/IL-9 family.

Cellular localization

Secreted.

Protocols