10X RIPA Buffer

Overview 10X RIPA Buffer AB156034-15ML

  • Tested applications

    Suitable for: WBELISASDS-PAGEIP
  • General notes

    Abcam’s 10X RIPA lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent effectively extracts cytoplasmic, nuclear and membrane proteins. It is compatible with many downstream applications, including SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.

    Preparation: Dilute to 1X in deionized water

     

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at Room Temperature. Store at Room Temperature.
  • Storage buffer

    pH: 7.50
    Constituents: 0.22% Beta glycerophosphate, 0.18% Sodium orthovanadate, 5% Sodium deoxycholate, 0.38% EGTA, 1% Sodium lauryl sulfate, 6.1% Tris, 0.29% EDTA, 8.8% Sodium chloride, 1.12% Sodium pyrophosphate decahydrate, 10% Nonylphenol, ethoxylated
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • RIPA

     

Application

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab156034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Suggested working concentration: 1X
ELISA
Use at an assay dependent concentration. Suggested working concentration: 1X
SDS-PAGE
Use at an assay dependent concentration. Suggested working concentration: 1X
IP
Use at an assay dependent concentration. Suggested working concentration: 1X