Human IL-15 ELISA Kit

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Key features and details

One-wash 90 minute protocol

Sensitivity: 1.1 pg/ml

Range: 3.9 pg/ml – 250 pg/ml

Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts

Detection method: Colorimetric

Assay type: Sandwich (quantitative)

Reacts with: Human

Overview

Product name

Human IL-15 ELISA Kit

Detection method

Colorimetric

Precision

Intra-assay
SamplenMeanSDCV%
Overall5  5.8%
Inter-assay
SamplenMeanSDCV%
Overall3  13%

Sample type

Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma

Assay type

Sandwich (quantitative)

Sensitivity

1.1 pg/ml

Range

3.9 pg/ml – 250 pg/ml

Recovery

 

Sample specific recovery
Sample typeAverage %Range
Serum9086% – 94%
Cell culture extracts9594% – 97%
Tissue Extracts10097% – 102%
Cell culture media110103% – 118%
Hep Plasma9290% – 95%
EDTA Plasma9592% – 97%
Cit plasma9794% – 103%

Assay time

1h 30m

Assay duration

One step assay

Species reactivity

Reacts with: Human
Does not react with: Cow

Product overview

Human IL-15 ELISA Kit (ab218266) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-15 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-15 with 1.1 pg/ml sensitivity.


SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


        – Single-wash protocol reduces assay time to 90 minutes or less
        – High sensitivity, specificity and reproducibility from superior antibodies
        – Fully validated in biological samples
        – 96-wells plate breakable into 12 x 8 wells strips


A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Notes

IL-15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. IL-15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL-15 and IL-2 share many biological properties and stimulatory activities (T, B, and NK cells). There is no sequence homology between IL-15 and IL-2, though computer modeling indicates both possess a four alpha helical bundle structure. IL-15 competes for binding sites with IL-2, as both IL-2 and IL-15 stimulate the growth of cells through the IL-2 receptor. IL-15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle.  

 

Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Platform

Pre-coated microplate (12 x 8 well strips)

Properties

Storage instructions

Store at +4°C. Please refer to protocols.
Components1 x 96 tests
10X Human IL-15 Capture Antibody1 x 600µl
10X Human IL-15 Detector Antibody1 x 600µl
10X Wash Buffer PT (ab206977)1 x 20ml
50X Cell Extraction Enhancer Solution (ab193971)1 x 1ml
5X Cell Extraction Buffer PTR (ab193970)1 x 10ml
Antibody Diluent CPI – HAMA Blocker (ab193969)1 x 6ml
Human IL-15 Lyophilized Recombinant Protein2 vials
Plate Seals1 unit
Sample Diluent NBP1 x 20ml
Sample Diluent NS (ab193972)1 x 50ml
SimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unit
Stop Solution1 x 12ml
TMB Development Solution1 x 12ml

Research areas

  • Immunology
  •  
  • Innate Immunity
  •  
  • Cytokines
  •  
  • Interleukins
  • Immunology
  •  
  • Immune System Diseases
  •  
  • Antiviral Signaling
  • Cancer
  •  
  • Tumor immunology
  •  
  • Cytokines
  •  
  • Interleukins
  • Immunology
  •  
  • Innate Immunity
  •  
  • NK Cells
  • Metabolism
  •  
  • Types of disease
  •  
  • Obesity
  • Neuroscience
  •  
  • Processes

Function

Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.

Tissue specificity

Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.

Sequence similarities

Belongs to the IL-15/IL-21 family.

Cellular localization

Secreted and Cytoplasm. Nucleus. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.

Protocols