Mouse Inflammation Antibody Array – Membrane (40 Targets)

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Overview

Product name

Mouse Inflammation Antibody Array – Membrane (40 Targets)
See all Inflammation Antibody Array antibody arrays

Sample type

Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media

Assay type

Semi-quantitative

Species reactivity

Reacts with: Mouse

Product overview

ab133999 is for simultaneous detection of 40 Mouse inflammatory factors. Suitable for all sample types.


Targets: BLC, CD30L, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CSF, IFN-gamma, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, I-TAC, KC/CXCL1, Leptin/OB, LIX, Lymphotactin, MCP-1, MCSF, MIG, MIP-1 alpha, MIP-1 gamma, RANTES, SDF-1, TCA-3, TECK, TIMP-1, TIMP-2, TNF-alpha, sTNF RI, sTNF-RII


Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

 

Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Tested applications

Suitable for: Multiplex Protein Detection

Properties

Storage instructions

Store at -20°C. Please refer to protocols.
Components1 x 4 Membranes1 x 8 Membranes
1,000X HRP-Conjugated Streptavidin1 x 50µl1 x 50µl
1X Blocking Buffer1 x 25ml2 x 25ml
20X Wash Buffer I1 x 10ml1 x 20ml
20X Wash Buffer II1 x 10ml1 x 20ml
2X Cell Lysis Buffer1 x 10ml1 x 16ml
8-Well Incubation Tray (with Lid)1 unit1 unit
Biotin-Conjugated Anti-Cytokines2 vials4 vials
Inflammation Antibody Array Membranes4 units8 units
Detection Buffer C1 x 1.5ml1 x 2.5ml
Detection Buffer D1 x 1.5ml1 x 2.5ml

Research areas

  • Immunology
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  • Innate Immunity
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  • Cytokines
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  • Interleukins
  • Immunology
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  • Innate Immunity
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  • Cytokines
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  • Interferons
  • Immunology
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  • Innate Immunity
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  • Chemokines
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  • Beta Chemokines (CC)
  • Antibody Arrays
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  • Membrane antibody arrays
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  • EGFR phosphorylation antibody arrays
  • Antibody Arrays
  •  
  • Membrane antibody arrays
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  • Inflammation antibody arrays

Protocols