Lipid Peroxidation (MDA) Assay Kit

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Key features and details

  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Adherent cells, Suspension cells, Tissue Lysate

Overview

Product name

Lipid Peroxidation (MDA) Assay Kit (Colorimetric)

Detection method

Colorimetric

Sample type

Adherent cells, Suspension cells, Tissue Lysate

Product overview

Lipid Peroxidation (MDA) Assay Kit (Colorimetric) ab233471 enables researchers to detect MDA without the heating steps required by the TBARS assay conventionally used for MDA detection.


In this MDA assay, the MDA Color Reagent reacts with MDA to generate a blue color product which is measured at 695 nm with absorbance microplate readers. The assay is very fast and specific for MDA with little interference from other aldehydes.


Alternatively, see our popular TBARS assay kit for MDA measurement ab118970.


MDA assay protocol summary for ab233471:
– add samples and standards to wells
– add MDA color reagent and incubate for 10-30 min at room temp
– add reaction solution and incubate for 30-60 min at room temp
– analyze with microplate reader 

Notes

Lipid peroxidation is characterized by the oxidative degradation of unsaturated fatty acids, phospholipids, glycolipids, cholesterol esters and cholesterol. Malondialdehyde (MDA) is one of the most commonly used biomarkers for lipid peroxidation.

Running an MDA assay has historically relied on a reaction with thiobarbituric acid (the TBARS assay) to generate a product that can be measured colorimetrically at 532 nm or fluorimetrically at Ex/Em = 530/550 nm.

However, the TBARS assay has quite a few limitations:
– the reaction is not specific to MDA,
– the TBA-MDA reaction needs be run under acidic conditions,
– the TBARS assay needs be run under high temperature, commonly at 90-100 ºC.

Properties

Storage instructions

Store at -20°C. Please refer to protocols.
Components200 tests
Dilution Buffer1 x 10ml
MDA Color Reagent1 vial
MDA Standard1 vial
Reaction Solution1 x 10ml

 

Protocols