Thromboxane B2 ELISA Kit

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Key features and details

  • Sensitivity: 10.54 pg/ml
  • Range: 13.7 pg/ml – 10000 pg/ml
  • Sample type: Hep Plasma, Saliva, Serum, Tissue Culture Media, Urine
  • Detection method: Colorimetric
  • Assay type: Competitive
  • Reacts with: Human

Overview

Product name

Thromboxane B2 ELISA Kit

Detection method

Colorimetric

Precision

Intra-assay
SamplenMeanSDCV%
Low24759pg/ml = 3.6%
Medium241283pg/ml = 4%
High242605pg/ml = 1.6%
Inter-assay
SamplenMeanSDCV%
Low844pg/ml = 7.6%
Medium8371pg/ml = 3.6%
High83053pg/ml = 6.2%

Sample type

Saliva, Urine, Serum, Tissue Culture Media, Hep Plasma

Assay type

Competitive

Sensitivity

= 10.54 pg/ml

Range

13.7 pg/ml – 10000 pg/ml

Recovery

 

Sample specific recovery
Sample typeAverage %Range
Saliva= 98.3% – %
Urine= 102.2% – %
Serum= 113% – %
Tissue Culture Media= 104.7% – %
Hep Plasma= 111% – %

Assay time

3h 00m

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Human
Predicted to work with: a wide range of other species

Product overview

Abcam’s Thromboxane B2 (TXB2in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thromboxane B2 in biological fluids.

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-TXB2 antigen and a rabbit polyclonal antibody specific to TXB2. After incubation the excess reagents are washed away and pNpp substrate is added. After a short incubation the enzyme reaction is stopped and a yellow color is generated. The intensity of the yellow coloration is inversely proportional to the amount of TXB2 captured in the plate.

Notes

Thromboxane A2 (TXA2) is involved in platelet aggregation, vasoconstriction and reproductive functions, but has a half life of only 37 seconds under physiological conditions. Thromboxane B2 (TXB2) is the stable product of the non-enzymatic hydration of TXA2, thus the production of TXA2 in vivo is typically monitored by measurement of TXB2 and its metabolite 2,3-dinor TXB2. 11-dehydro-TXB2 is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma produced by its dehydrogenation by the enzyme 11-OH-dehydrogenase.

Cross Reactivity 

Compound  Cross Reactivity
 TXB2 100%
 2,3-dinorTXB2 7.1%
 11-dehydro TXB2 0.4%
 6-keto-PGF 0.23%
 PGD2 <0.1%
 PGE2<0.1%
 PGF <0.1%
 PGF  <0.1%

Platform

Microplate

Properties

Storage instructions

Store at +4°C. Please refer to protocols.
Components1 x 96 tests
20X Wash Buffer Concentrate1 x 27ml
Assay Buffer1 x 27ml
Goat anti-rabbit IgG Microplate (12 x 8 wells)1 unit
Plate Sealer1 unit
pNpp Substrate1 x 20ml
Rabbit Polyclonal Thromboxane B2 Antibody1 x 5ml
Stop Solution1 x 5ml
Thromboxane B2 Alkaline Phosphatase Conjugate1 x 5ml
Thromboxane B2 Standard1 x 500µl

Research areas

  • Signal Transduction
  •  
  • Signaling Pathway
  •  
  • Lipid Signaling
  •  
  • Thromboxane
  • Kits/ Lysates/ Other
  •  
  • Kits
  •  
  • ELISA Kits
  •  
  • ELISA Kits
  •  
  • Eicosanoid ELISA Kits

Alternative names

  • T box 2
  • T box transcription factor TBX2
  • T-box protein 2
  • T-box transcription factor TBX2
  • Thromboxane B2

Protocols