Soluble Collagen Assay Kit

SKU: N/A Categories: ,

Key features and details

  • Detection method: Fluorescent

Overview

Product name

Soluble Collagen Assay Kit

Detection method

Fluorescent

Product overview

Soluble Collagen Assay Kit (ab241015) detects newly synthesized (acid-soluble) collagen levels in various biological samples and cultured cell medium.


The assay involves extraction of soluble collagen in acetic acid, followed by enzymatic degradation of collagen into glycine-rich oligopeptides, which are quantified using a fluorogenic reagent and developer solution that selectively react with the N-terminal glycine fragments to form a stable fluorescent complex (Ex/Em = 376/468 nm).


The assay is more sensitive and selective than dye-binding (Picrosirius Red) soluble collagen assays, is simple to perform and has a linear range from 0.05 – 2 μg collagen per well (2.5 μg/mL to 100 μg/mL in a 20 μL sample volume).

Notes

This product is manufactured by BioVision, an Abcam company and was previously called K532 Soluble Collagen Assay Kit (Fluorometric). K532-100 is the same size as the 100 test size of ab241015.

Properties

Storage instructions

Store at -20°C. Please refer to protocols.
Components100 tests
Collagen Assay Buffer1 x 25ml
Collagen I Standard (2 mg/ml)1 x 100µl
Collagenase Enzyme Mix1 vial
Detection Reaction Buffer1 x 10ml
Developer Solution1 x 500µl
Peptide Labelling Reagent1 vial

Protocols