E. coli HCP ELISA Kit (host cell protein)

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Key features and details

  • Sensitivity: 30 ng/ml
  • Range: 3 ng/ml – 810 ng/ml
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)

Overview

Product name

E. coli HCP ELISA Kit (host cell protein)

Detection method

Colorimetric

Assay type

Sandwich (quantitative)

Sensitivity

30 ng/ml

Range

3 ng/ml – 810 ng/ml

Assay time

3h 00m

Assay duration

Multiple steps standard assay

Product overview

E. coli HCP ELISA Kit (host cell protein) (ab240997) is designed to quantitatively measure host cell protein (HCP) contamination in bulk products expressed in E. coli expression systems.


The provided 96-well plate has been pre-coated with Rabbit anti-E. coli HCP antibodies to capture E. coli proteins from biological samples. Following an incubation to allow capture of the E. coli protein by the antibodies on the plate, a second anti- E. coli HCP antibody, conjugated with biotin, is added and incubated to allow it to bind to the captured E. coli proteins. After incubation, the plate is washed and a Streptavidin-HRP conjugate is added and incubated for 30 min. The Streptavidin-HRP conjugate will be captured by any biotin labeled antibody bound to the plate. Following a wash step to remove unbound conjugate, TMB substrate is added and is converted by the captured HRP to a colored product in proportion to the amount of HCP bound to the plate. After a short incubation to allow color development, the reaction is stopped and the intensity of the generated color is detected in a spectrophotometer plate reader capable of measuring 450nm wavelength.

Platform

Pre-coated microplate (12 x 8 well strips)

Properties

Storage instructions

Store at +4°C. Please refer to protocols.
Components1 x 96 tests
E. coli Protein Standard1 x 600µl
10X PBST1 x 30ml
5X Dilution Buffer1 x 15ml
Coated Clear 96 Well Plates1 unit
Plate Sealer1 unit
Reporting Antibody1 x 150µl
Stop Solution1 x 15ml
Streptavidin-HRP Conjugate1 x 400µl
TMB Substrate1 x 15ml

Research areas

  • Microbiology
  •  
  • Organism
  •  
  • Bacteria
  •  
  • Gram negative Bacteria
  •  
  • Escherichia coli

Relevance

Escherichia coli is a bacterium that is commonly found in the lower intestine of warm-blooded animals.

Alternative names

  • Escherichia coli

Protocols