Human Anti-Toxocara canis IgG ELISA Kit

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Key features and details

  • Sample type: Cit plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Indirect
  • Reacts with: Human

Overview

Product name

Human Anti-Toxocara canis IgG ELISA Kit

Detection method

Colorimetric

Precision

Intra-assay
SamplenMeanSDCV%
Pos Serum12  2.6%
Inter-assay
SamplenMeanSDCV%
Pos. Serum5  8.1%

Sample type

Serum, Hep Plasma, Cit plasma

Assay type

Indirect

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Human

Product overview

Abcam’s anti-Toxocara canis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Toxocara canis in Human serum and plasma.


A 96-well plate has been precoated with Toxocara canis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled Protein A conjugate is added to the wells, which binds to the immobilized Toxocara canis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Toxocara canis IgG sample captured in plate.

Platform

Microplate

Properties

Storage instructions

Store at +4°C. Please refer to protocols.
ComponentsIdentifier1 x 96 tests
20X Washing SolutionWhite cap1 x 50ml
Cover foil 1 unit
IgG Sample DiluentYellow, White cap1 x 100ml
Stop Solutionred cap1 x 15ml
Strip holder 1 unit
TMB Substrate SolutionYellow cap1 x 15ml
Toxocara canis Coated Microplate (12 x 8 wells)12 strips of 8 wells1 unit
Toxocara canis Cut-off ControlYellow, Green cap1 x 3ml
Toxocara canis Negative ControlYellow, Blue cap1 x 2ml
Toxocara canis Positive ControlYellow, Red cap1 x 2ml
Toxocara canis Protein A HRP ConjugateBlue, Black cap1 x 20ml

Research areas

  • Immunology
  •  
  • Immunoglobulins
  •  
  • Heavy Chain
  •  
  • IgG
  • Microbiology
  •  
  • Organism
  •  
  • Eukaryotic Microorganism
  •  
  • Metazoa
  •  
  • Nematoda
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  • ELISA Kits
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  • Pathogen specific immunoglobulins ELISA kits
  • Kits/ Lysates/ Other
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  • Infectious disease ELISA kits

Protocols