AGE (Advanced Glycation End) Assay Kit

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Key features and details

  • Assay type: Competitive
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Cell Lysate, Plasma, Purified protein, Serum, Tissue Lysate
  • Sensitivity: 0.5 µg/ml

Overview

  • Product name

    AGE (Advanced Glycation End) Assay Kit
    See all AGE kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma, Cell Lysate, Purified protein, Tissue Lysate
  • Assay type

    Competitive
  • Sensitivity

    0.5 µg/ml
  • Range

    0.36 µg/ml – 100 µg/ml
  • Product overview

    AGE (Advanced Glycation End Products) Assay Kit (ab238539) is designed for the rapid detection and quantitation of advanced glycation end product protein adducts.


    Advanced Glycation End Products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent advanced glycation end products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The quantity of AGE adduct in protein samples is determined by comparing its OD with that of a known AGE-BSA standard curve.

  • Platform

    Microplate reader

Properties

Storage instructions

Please refer to protocols.
Components96 tests
100X Conjugate Diluent1 x 300µl
10X Wash Buffer1 x 100ml
AGE Conjugate1 x 50µl
AGE-BSA Standard1 x 125µl
Anti-AGE Antibody (1000X)1 x 10µl
Assay Diluent1 x 50ml
Protein Binding Strip Well Plate1 unit
Secondary Antibody, HRP Conjugate (1000X)1 x 20µl
Stop Solution1 x 12ml
Substrate Solution1 x 12ml

Research areas

  • Immunology
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  • Immune System Diseases
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  • Autoimmune
  • Signal Transduction
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  • Metabolism
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  • Energy Metabolism
  • Cardiovascular
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  • Atherosclerosis
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  • Lipoprotein metabolism
  • Cardiovascular
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  • Atherosclerosis
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  • Diabetes associated
  • Cancer
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  • Cancer Metabolism
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  • Metabolic signaling pathway
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  • Metabolism of lipids and lipoproteins
  • Cancer
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  • Cancer Metabolism
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  • Metabolic signaling pathway
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  • Integration of energy metabolism
  • Metabolism
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  • Pathways and Processes
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  • Metabolic signaling pathways
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  • Lipid and lipoprotein metabolism
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  • Lipid metabolism
  • Metabolism
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  • Pathways and Processes
  •  
  • Metabolic signaling pathways
  •  
  • Lipid and lipoprotein metabolism
  •  
  • Lipoprotein metabolism
  • Metabolism
  •  
  • Pathways and Processes
  •  
  • Metabolic signaling pathways
  •  
  • Energy transfer pathways
  •  
  • Energy Metabolism
  • Metabolism
  •  
  • Pathways and Processes
  •  
  • Metabolic signaling pathways
  •  
  • Energy transfer pathways
  •  
  • Integration of energy
  • Metabolism
  •  
  • Types of disease
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  • Diabetes
  • Metabolism
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  • Types of disease
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  • Heart disease

Relevance

The non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure.

Cellular localization

Cell Membrane and Secreted

Alternative names

  • Advanced glycation end products
  • AGEs

Protocols