Substance P ELISA Kit

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Key features and details

  • Sensitivity: 5.3 pg/ml
  • Range: 9.8 pg/ml – 10000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Competitive
  • Reacts with: Species independent

Overview

Product name

Substance P ELISA Kit
See all Substance P kits

Detection method

Colorimetric

Sample type

Cell culture supernatant, Saliva, Urine, Serum, Plasma

Assay type

Competitive

Sensitivity

5.3 pg/ml

Range

9.8 pg/ml – 10000 pg/ml

Recovery

 

Sample specific recovery
Sample typeAverage %Range
Saliva108.3% – %
Urine105.8% – %
Serum97.7% – %
Tissue Culture Media81% – %
Hep Plasma109.3% – %

Assay time

3h 00m

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Species independent

Product overview

Abcam’s Substance P in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Substance P in biological fluids.


A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Substance P antigen and a polyclonal rabbit antibody specific to Substance P. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Substance P captured in the plate.

Notes

Substance P is a undecapeptide that displays a number of biological activities. The peptide was first discovered in 1931 by von Euler and Gaddum. They reported that extracts of equine brain and intestine contained a hypotensive and spasmogenic factor. The preparation, termed preparation P, was later found to be proteinaceous. The isolation and characterization of Substance P was carried out by Leeman’s group in 1970. The structure is shown below.

Substance P:  H-Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2

Substance P is synthesized in the ribosomes as a larger protein and then enzymatically converted into the active peptide. The peptide is widely distributed in the peripheral and central nervous systems of vertebrates, where it is thought to act as a neurotransmitter. In the peripheral system, Substance P is localized in the primary sensory neurons and neurons intrinsic to the gastrointestinal tract.

Cross Reactivity

Compound% Cross Reactivity
Substance P100
Substance P (4-11)>100
Substance P (7-11)>100
Substance P (3-11)66
Physalaemin50
Eledoisin<0.001
α-Neurokinin<0.001
β-Neurokinin<0.001
Somatostatin<0.001
Substance P (1-4)<0.001

Platform

Microplate

Properties

Storage instructions

Please refer to protocols.
Components1 x 96 tests
20X Wash Buffer Concentrate1 x 27ml
Assay Buffer1 x 27ml
Goat anti-rabbit IgG Microplate (12 x 8 wells)1 unit
Plate Sealer1 unit
pNpp Substrate1 x 20ml
Stop Solution1 x 5ml
Substance P Alkaline Phosphatase Conjugate1 x 5ml
Substance P Antibody1 x 5ml
Substance P Standard1 x 500µl

Research areas

  • Neuroscience
  •  
  • Neurotransmitter
  •  
  • Neuropeptides
  •  
  • Tachykinins
  • Neuroscience
  •  
  • Neurotransmitter
  •  
  • Neuropeptides
  •  
  • Hormones
  • Neuroscience
  •  
  • Sensory System
  •  
  • Somatosensory system
  •  
  • Nociception
  • Neuroscience
  •  
  • Neurology process
  •  
  • Metabolism
  • Neuroscience
  •  
  • Processes

Function

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

Sequence similarities

Belongs to the tachykinin family.

Cellular localization

Secreted.

Protocols